β-Hexosaminidase Activity Assay, 100 Tests

β-Hexosaminidase are hydrolytic enzyme responsible for the breakdown of carbohydrates. Specifically, β-Hexosaminidase cleave the terminal β-D-glucuronic acid residue from the nonreducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Hexosaminidase activity results in metabolic disease and health problems. It is important to detect β-Hexosaminidase activity in the tested samples for disease examination.

The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.

Applications:

This kit is used for the determination of hexosaminidase activity in biological samples.

Key features:

• Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30 minutes reaction at 37˚C.
• High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Storage Conditions:

This kit is shipped on ice. Store all components at -20℃ in a non-frost-free freezer. Shelf life of 12 months after receiving.