Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) Solution is an extraordinarily powerful, odorless, thiol-free reducing agent with broad application to protein and nucleic acid involving reduction of disulfide bonds. The pH value of the solution is designed at 6.0, 7.0, and 8.0 for multiple choices. This product is an effective and convenient replacement for βmercaptoethanol (β-ME) or DTT in protein analysis.
The product is supplied as a 0.5M solution at pH 6.0, 7.0, and 8.0.
TCEP breaks disulfide bonds and can be used for a wide variety of protein applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. Its ability to prevent oxidation of protein samples makes it a useful buffer component because it helps to preserve enzymatic activity.