2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination.
|Fidelity (vs. Taq)||1 ×|
|Polymerase||Taq DNA Polymerase|
|Reaction Format||SuperMix or Master Mix|
|Product Type||PCR Master Mix (2×)|
|10102||2×HieffTM PCR Master Mix (With Dye)||1 mL||5×1 mL||50×1 mL||100×1 mL|
The 2×Hieff™ PCR Master Mix products should be stored at -15℃ ~ -25℃ for 2 years.
Figure1. The expected 1.2 kb PCR products can be amplified with 2×HieffTM PCR Master Mix.
The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.