2x Fast Sybr Green qPCR Master Mix with different ROX levels and package sizes provide a convenient choice suitable for different qPCR instruments. It is a 2x concentrated premix containing Taq DNA polymerase, dNTPs, Mg2+, SYBR Green I dye (detection), ROX reference dye, and PCR buffer components. The proprietary chemical modifications of the enzyme make PCR hot start activation much faster (less than 1 minute) than the normal 10-minute activation in standard real-time qPCR amplifications. This confers a significant reduction in non-specific PCR amplification (see Figure 1). As the enzyme buffer system is optimized for real-time PCR, the product offers high amplification efficiency and high detection sensitivity in real-time PCR.
This kit can be used for real-time PCR amplification.
|Kit Catalog||Description||Kit Size||qPCR Instrument|
|TBS4001R-2.5||2x Fast SyBr Green qPCR Master Mix ROX||2x 1.25mL||ABI® PRISM® 7000, 7700, 7900HT, ABI® 7300 qPCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™|