ATP, Adenosine-5'-triphosphate, and its sodium salt are the basic components of energy change in many biological systems and can be used in a variety of molecular biology applications, such as kinase assays, in vitro transcription, ligation and phosphorylation reactions, etc.
This product is a transparent colorless aqueous solution prepared from ATP trisodium salt, and free from DNase, RNase and phosphatase contamination.
This product is produced in accordance with GMP process requirements and provided in liquid form.
|CAS No||56-65-5 (free acid)|
|Molecular Weight||573.13 g/moL|
|Content||100 mM ± 3 mM|
|10129||ATP Solution GMP-grade (100 mM)||1 mL|
The product should be stored at -25℃ ~ -15℃ for two years.
Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.
Figure 2. Synthesis of capped RNA in vitro.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads. The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.