TBST (Tris Buffered Saline with Tween-20) is a isotonic buffered saline solution commonly used in biology. It is a solution with a certain proportion of Tween-20 added to TBS. It is mainly used for the preparation of blocking solution, preparation of primary and secondary antibodies, and washing after incubation of primary and secondary antibodies in experiments such as immunoblotting (WB), immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (IC). Tween-20, as a detergent, can increase the elution capacity of the buffer.
Composition
Tris |
10 mM |
NaCl |
150 mM |
Tween-20 |
0.05%(V/V) |
Product Properties
Appearance |
Colorless clear liquid |
pH |
7.5±0.1 |
Storage and Shelf Life
Store at room temperature (2-30 ℃), valid for 2 years.
Ordering Information
Cat # | Description | Formula | Package |
TBST-500 | TBST pH7.5 buffer(1×) |
150 mM NaCl, 10 mM Tris,0.05% (V/V) Tween-20 | 500mL/ Bottle, 10 Bottles/Case |
TBST-500-1 | TBST pH7.5 buffer(1×) |
150 mM NaCl, 10 mM Tris,0.05% (V/V) Tween-20 | 500mL/ Bottle |