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CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)
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CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

SKU BLG-10628ES10
$214.89
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CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)
Product Details
Weight: 5.00 lbs

Description:

This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

Note: G* is the first base of the RNA transcript.

Feature

  • dsRNA level lower to about 1/100000
  • Comptiable with Trilink CleanCap AG
  • High yields comparable to WT
  • Lower Cap input
  • Animal origin-free (AOF)

Specifications:

Source

Recombinant E. coli with T7 RNA Polymerase gene

Optimum Temperature

37℃

Storage Buffer

50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100,50% (v/v) glycerin,pH7.9 at 25℃

Unit Definition

The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.

Recommended Mg2+

30mM Magnesium Acetate

QC Standard:

Items

Specification/Standard

Enzyme activity

250-300U/μL

Protein Purity

≥95%

Endotoxin

<20 EU/mg

Protease

Negative

Exonuclease

Negative

Nickase

Negative

RNase

Negative

E.coli host protein

<50ppm

E.coli host DNA

<10 fg/U

Mycoplasma examination

Negative

Shipping and Storage:

The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

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