This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.
Note: G* is the first base of the RNA transcript.
Feature
|
Source |
Recombinant E. coli with T7 RNA Polymerase gene |
|
Optimum Temperature |
37℃ |
|
Storage Buffer |
50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100,50% (v/v) glycerin,pH7.9 at 25℃ |
|
Unit Definition |
The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit. |
|
Recommended Mg2+ |
30mM Magnesium Acetate |
|
Items |
Specification/Standard |
|
Enzyme activity |
250-300U/μL |
|
Protein Purity |
≥95% |
|
Endotoxin |
<20 EU/mg |
|
Protease |
Negative |
|
Exonuclease |
Negative |
|
Nickase |
Negative |
|
RNase |
Negative |
|
E.coli host protein |
<50ppm |
|
E.coli host DNA |
<10 fg/U |
|
Mycoplasma examination |
Negative |
Shipping and Storage:
The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

