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DMEM/F-12 contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture. DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, and it combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 is widely used for supporting the growth of many different mammalian cells, including MDCK, glial cells, fibroblasts, human endothelia cells, and rat fibroblasts. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. All raw material components used for Hygia’s cell culture media products are screened through using strict quality control testing. Hygia cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media is subjected to sterility test, pH, osmolality, and cell culture test.