DMEM/F12, HEPES

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and Ham’s F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, is enriched with Ham’s Nutrient Mixture F-12’s additional components including HEPES, sodium pyruvate, glutamine, and glucose.

DMEM/F-12 is widely used for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

APPLICATION

• MDCK cell
• Glial cells
• Fibroblasts
• Chondrocytes
• Human epithelial cells
• Lung cells