Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric)

TBS2088-200
$269.00
In stock
1
Product Details

Glucose oxidase (GOD) catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.

Tribioscience’s Fast Glucose Oxidase Activity Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay.

Applications:

Direct Assay: Glucose oxidase activity in serum, plasma, urine, and other bio-samples.

Key Features:

Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50μL samples. Detection ranges 0.1-50 μM in 96-well plate for colorimetric assay and 0.002-10 μM for fluorometric assay.
Simple and High-Throughput: One-step procedure. Load-Incubate-Read. The kit can be used for a robust method.
Time Saving: takes less than 30 minutes.

Kit Contents:

Component TBS2088-200 TBS2088-600
Assay Buffer 1x 12mL 3x 12mL
Red Probe 1x 0.25mL 1x 0.8mL
GOD Standard Stock (1 U/mL) 1x 25μL 1x 80μL
Enzymes 1x 2mL 3x 2mL
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