Tribioscience’s Fast Human Phospho Tau Thr181 (p-Tau-181) ELISA is designed to quantitatively detect human p-Tau-181 levels in serum, plasma, CSF, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau-181 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau-181 protein.
This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
This assay recognizes natural and recombinant human pTau-181. No cross-reactivity with others.