HBSS (Hanks’ Balanced Salt Solution) is used for a variety of cell culture applications, including washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. <br>Hygia’s balanced salt solutions are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of HBSS solutions are subjected to sterility test, pH, endotoxin, and osmolality.
HBSS with calcium and magnesium is generally used as transport media or for reagent preparation.
HBSS without calcium and magnesium is required for rinsing chelators from the culture before cell dissociation.
Overview | ||
HBSS with Ca and Mg |
HBSS without Ca and Mg |
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Composition | Glucose, Calcium, Magnesium |
Glucose |
Does NOT contain | Phenol red | Calcium, Magnesium, Phenol red |
pH | 6.7-7.8 at 25℃ | 6.7-7.8 at 25℃ |
Storage/shipping condition |
Room temperature | Room temperature |
Shelf life | 24 months from date of manufacture |
24 months from date of manufacture |