Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours.
Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections.
|Cell Type||Established Cell Lines|
|Sample Type||Plasmid DNA, Synthetic siRNA|
|Transfection Technique||Lipid-Based Transfection|
|40802||Hieff TransTM;Liposomal Transfection Reagent||0.5 mL||1 mL||5×1 mL|
The product is shipped with ice packs and can be stored at 2-8ºC for one year. Do not freeze!
Figure 1. Hieff Trans™ Liposome Transfection Reagent outperforms the transfection reagent from the competitive brand（T* brand in Figure 1a, P* brand in Figure 1b).
Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products.
|Product Name||Hieff TransTMLiposomal Transfection Reagent|
|Validated Cell Lines||293T||Calu 1||HEK293||HO1980||N2A||TS|
Figure 2. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update).
Cited from "Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. doi: 10.1038/s41587-021-01112-1"
Cited from "Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1;81(13):2765-2777.e6. doi: 10.1016/j.molcel.2021.05.010."
Cited from "UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16;109(12):1949-1962.e6. doi: 10.1016/j.neuron.2021.04.023."