For for the preservation and cultivation of Escherichia coli in molecular biology experiments.
PREPARATION
Suspend 40.0 g in 1 L of distilled or deionized water. Dispense and autoclave it at 121 ℃ for 15min.
PRINCIPLE
Tryptone and yeast extract provide carbon source, nitrogen source, vitamins and minerals. Sodium chloride maintains a balanced osmotic pressure. Agar is the coagulant of culture medium.
COMPOSITION
Composition |
Amount(g/L) |
Tryptone |
10.0 |
Yeast Extract |
5.0 |
Sodium Chloride |
10.0 |
Agar |
15.0 |
Final pH (25 ℃) |
7.0 ±0.2 |
STORAGE
Store in a dry place away from light, tighten the cap immediately after use.