For the preservation and cultivation of Escherichia coli in molecular biology experiments. (Molecular cloning experiment guide)
PREPARATION
Suspend 25.0 g in 1 L of distilled or deionized water. Dispense and autoclave it at 121 ℃ for 20min.
PRINCIPLE
Tryptone and yeast extract provide carbon source, nitrogen source, vitamins and minerals. Sodium chloride maintains a balanced osmotic pressure.
COMPOSITION
Composition |
Amount(g/L) |
Tryptone |
10.0 |
Yeast Extract |
5.0 |
Sodium Chloride |
10.0 |
Final pH (25 ℃) |
7.0 ±0.2 |
STORAGE
Store in a dry place away from light, tighten the cap immediately after use.