MCDB 153 medium with Trace Elements, L-Glutamine, and Sodium Pyruvate, 500 ml

MCDB media were developed for the culture of specific cell types without a serum supplement. MCDB 153 medium is a modification of Ham’s nutrient mixture F-12 designed specifically for the growth of human epidermal keratinocyte culture, clonal growth of chicken embryo fibroblasts, or Chinese Hamster Ovary (CHO) cells.

MCDB 153 medium includes trace elements, L-glutamine, sodium pyruvate, and HEPES for more effective buffering capacity and energy sources. The details are listed in the formulation. The main modifications are below:

• L-Glutamine
• HEPES
• Sodium Pyruvate
• Phenol Red
• Sodium Bicarbonate
• Insulin, transferrin, and sodium selenite
• Glucose

APPLICATION

• Human epidermal keratinocyte
• Chinese Hamster Ovary (CHO)