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MEM (Minimum Essential Medium) is one of the most commonly used basal mediums for supporting the growth of many suspension and adherent mammalian cells. MEM medium is a more nutrient rich version of basal medium eagle (BME) media, and is suitable for Hela, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts and primary rat astrocytes. There are some other modifications of MEM followed, including with or without non-essential amino acids (including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine, and Glycine), addition of ribonucleosides and deoxyribonucleosides, with Earle’s salts for use in a CO2 incubator or with Hank’s salts for use without CO2. This product is made with Earle’s salts, includes non-essential amino acids, no addition of ribonucleosides and deoxyribonucleosides. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. All raw material components used for Hygia’s cell culture media products are screened through using strict quality control testing. Hygia cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media is subjected to sterility test, pH, osmolality, and cell culture test.