RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.
|Source||Recombinant E.coli with Murine RNase inhibitor gene|
|Storage Buffer||20 mM Hepes KOH，150 mM KCl，8 mM DTT，0.5%（v/v）NP 40，0.5%（v/v）Tween20，50%（v/v）Glycerol，pH 7.5 ±0.2 (25℃)|
|Unit Definition||The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit|
|Components No.||10621ES10 (10 KU)||10621ES20 (20 KU)||10621ES60 (100 KU)||10621ES99 (1 MU)|
|BLG-10621ES10||250 μL||500 μL||2.5 mL||25 mL|