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Pseudo UTP Tris Solution GMP-grade (100 mM) 100 μL 1 mL

Pseudo UTP Tris Solution GMP-grade (100 mM) 100 μL 1 mL

SKU BLG-10656ES70/BLG-10656ES80
$639.39
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Pseudo UTP Tris Solution GMP-grade (100 mM) 100 μL 1 mL
Product Details
Weight: 5.00 lbs

Description

Pseudouridine-5-triphosphate solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

  • Validated, product-specific processes and analytical methods
  • Product-specific stability
  • Documentation follows applicable GMP guidelines
  • AOF production process and raw materials (TSE & BSE)
  • Nitrosamine statement
  • Regulatory support documents available
  • Large-scale production
  • The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system

Application

  • RNA synthesis and amplification
  • Building block for in vitro transcription

Specification

CAS No. 1175-34-4 (free acid)
Molecular formula C9H15N2O15P3 (free acid)
Molecular weight 484.14 g/mol (free acid)
Purity ≥ 99%
Content 100 mM ± 3 mM
Structure

Component

Components No. Name 10656ES70 10656ES80
10656 Pseudo UTP Tris Solution GMP-grade (100 mM) 100 μL 1 mL

Storage

The product should be stored at -25℃ ~ -15℃ for two years.

Figures

  • Increased the IVT yield

Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.

  • Decreased the dsRNA content

Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by Dot Blot method.

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