RPMI 1640 Medium was developed by Moore and his co-workers in 1966 at Roswell Park Memorial Institute, hence the acronym RPMI. It is a modification of McCoy′s 5A Medium. This medium was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells.