T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.
This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.
Figure 1: In vitro RNA transcription process
|Components No.||Name||10623ES50 (50 T)|
|10623-A||T7 RNA Polymerase Mix||100 μL|
|10623-B||10×Transcription Buffer||100 μL|
|10623-C||ATP (100mM)||100 μL|
|10623-D||CTP (100mM)||100 μL|
|10623-E||GTP (100mM)||100 μL|
|10623-F||UTP (100mM)||100 μL|
|10623-G||Control DNA Template (500ng/μL)||10 μL|
Dry ice transportation. Store at -15℃ ~ -25℃, valid for two years.