Transformation of E. coli with pGAL™ (Blue Colony)

In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

No IPTG used in this experiment.

• Explore genetic engineering, the central dogma, and enzymes in this packed experiment

• Transform E.coli with a plasmid DNA containing β-galactosidase and β-lactamase genes

• Identify transformed cells based on their blue color and ability to survive on agar with ampicillin