Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.
|Polymerase||Taq DNA polymerase|
|Type of sample||DNA|
|Application equipment||Most qPCR instruments|
|Product Type||SYBR premix for real-time fluorescence quantitative PCR|
|Apply to (application)||Gene Expression|
|11184||Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based)||1 mL||5×1 mL|
The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.