CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

Description:

This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

Note: G* is the first base of the RNA transcript.

Feature

• dsRNA level lower to about 1/100000
• Comptiable with Trilink CleanCap AG
• High yields comparable to WT
• Lower Cap input
• Animal origin-free (AOF)

Specifications:

QC Standard:

Shipping and Storage:

The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.