Universal Blue qPCR Master Mix (SYBR Green Based)

Description

Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.

Features

• Built-in visual indicator: It contains an inert blue dye that does not interfere with real-time PCR and provides higher visibility in tubes or Wells.
• High specificity: It contains Taq DNA polymerase and is tightly controlled by an antibody-mediated heat-priming mechanism, thereby avoiding nonspecific amplification.
• Wide instrument compatibility: It is compatible with most qPCR instruments and eliminates the need to adjust the concentration of ROX on different instruments

Applications

• Gene Expression

Specifications

Components

Shipping and Storage

The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.